Code
NGS-MPLX-01BX
Test
Active-Compendium
81339
Clinical
Oncology,Pathology
<p><b>Bone Marrow Aspirate:</b> 1-2 mL in EDTA tube. Sodium Heparin is acceptable.</br>
<b>Peripheral Blood:</b> 3-5 mL in EDTA tube. Sodium Heparin is acceptable.</br>
<b>Note:</b> Test is DNA-based. Please select Extract & Hold - DNA if specimen hold service is desired.</p>
<b>Peripheral Blood:</b> 3-5 mL in EDTA tube. Sodium Heparin is acceptable.</br>
<b>Note:</b> Test is DNA-based. Please select Extract & Hold - DNA if specimen hold service is desired.</p>
Flag Active
True
Orderable Test Description
<p>Next-generation sequencing of exon 10 of the MPL gene to detect all possible mutations, including those at the W515 and S505 codons. This test may be ordered separately (if JAK2 analysis, specifically JAK2 V617F, is already performed), as a reflex from JAK2 V617F Mutation Analysis, or as part of the MPN JAK2 V617F with Sequential Reflex to JAK2 Exon 12-15, CALR, and MPL.</br></br>
<b>Note:</b> This assay replaces the former MPL Mutation Analysis performed by bi-directional sequencing. NY samples are still processed by bi-directional sequencing until further notice.</p>
<b>Note:</b> This assay replaces the former MPL Mutation Analysis performed by bi-directional sequencing. NY samples are still processed by bi-directional sequencing until further notice.</p>
Orderable Turn Around Time
7 Days
Keywords
Orderable Biomarkers
NY Approved
False
Orderable Biomarkers JSON
{"DNA Sequencing": {"SNVs + Indels": ["MPL"]}}
Keywords string
MPL Mutation, mpl MPL Mutation Analysis by NGS
Title URL
mpl-mutation-analysis-by-ngs
Clinical Significance
Exon 10 MPL mutations, most commonly W515 and S505, are present in approximately 5-10% of patients with primary myelofibrosis (PMF) and essential thrombocythemia (ET). MPL and CALR mutations have been associated with increased risk of myelofibrotic transformation in ET cases. (References: PMID: 36347013)
Storage and transportation
Use cold pack for transport, making sure cold pack is not in direct contact with specimen. For fresh samples: refrigerate before shipping and ship same day as drawn whenever possible. Do not freeze.