IgVH Mutation Analysis

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Code
MOG-IVHX-01BX
Test
Active-Compendium
81263
Clinical
Oncology,Pathology
<p><b>Bone Marrow Aspirate:</b> 2 mL in EDTA tube.<br>
<b>Peripheral Blood:</b> 5 mL in EDTA tube.<br>
<b>Note:</b> Sodium Heparin tubes are acceptable.<br>
<b>Note:</b> Test is RNA-based. Please select Extract & Hold - RNA if specimen hold service is desired.</p>
Hematologic Diseases
Chronic lymphocytic leukemia (CLL)/Small lymphocytic lymphoma (SLL)
Molecular
Global
Flag Active
True
Orderable Test Description
This test uses RT-PCR and bi-directional sequencing of the variable region of the immunoglobulin heavy chain to detect mutations from the germline sequence. Positive reports identify the mutated VH gene family when there is >2% sequence deviation. Mutation may not be detectable in specimens with low tumor burden or high background signal.
Orderable Turn Around Time
10 Days
Keywords
igvh
igvh mutation
Meta Description
<p>RT-PCR and bi-directional sequencing of the variable region of the immunoglobulin heavy chain for detection of mutation from germline sequence. The mutated VH gene family is identified in positive reports (3% sequence deviation). Mutation may not be detectable in specimens containing 10% clonal B-cells.</p>
Orderable Biomarkers
IgVH
NY Approved
True
Orderable Biomarkers JSON
{"DNA Sequencing": {"Genomic Signatures": ["IgVH"]}}
Keywords string
igvh, igvh mutation IgVH Mutation Analysis
Title URL
igvh-mutation-analysis
Clinical Significance

Immunoglobulin heavy chain gene variable region (IgVH) somatic mutation status provides important prognostic information for patients with chronic lymphocytic leukemia (CLL), small lymphocytic lymphoma (SLL), and mantle cell lymphoma (MCL).

In studies, median survival for patients with CLL/SLL unmutated IgHV region gene was 6-9 years whereas median survival for patients with hypermutated IgHV region was 13-24 years. Mutated MCL patients have a better outcome compared to unmutated IGHV patients, with 5-year overall survival (OS) of 59% and 40%, respectively.

Storage and transportation
Use cold pack for transport, making sure cold pack is not in direct contact with specimen. Ship same day as drawn whenever possible. NYS clients please provide date and time of collection. Please select Extract & Hold - RNA if specimen hold service is desired.