Code
FSH-TCLO-01BX
Test
Active-Compendium
88377x1 manual or 88374x1 automated.
Clinical
Pathology
<p><b>Bone Marrow Aspirate:</b> 1-2 mL in sodium heparin tube. EDTA tube is acceptable.<br>
<b>Peripheral Blood:</b> 2-5 mL in sodium heparin tube. EDTA tube is acceptable.<br>
<b>Fresh, Unfixed Tissue:</b> Tissue in RPMI.<br>
<b>Fluids:</b> Mix in equal parts with RPMI (specimen volume to RPMI ratio of 1:1).<br>
<b>Fixed Cell Suspension:</b> A client-prepared fixed cell suspension is acceptable if received in 3:1 Methanol:Glacial Acetic Acid.<br>
<b>H&E slide (required) plus paraffin block.</b><br>
<b>Cut Slides:</b> H&E slide (required) plus 3 unstained slides cut at 4-5 microns.<br>
<b>Note for Tech Only clients:</b> Please circle H&E slide.<br>
<b>Note for NYS clients:</b> Cut slides and paraffin block specimens are not accepted.</p>
<b>Peripheral Blood:</b> 2-5 mL in sodium heparin tube. EDTA tube is acceptable.<br>
<b>Fresh, Unfixed Tissue:</b> Tissue in RPMI.<br>
<b>Fluids:</b> Mix in equal parts with RPMI (specimen volume to RPMI ratio of 1:1).<br>
<b>Fixed Cell Suspension:</b> A client-prepared fixed cell suspension is acceptable if received in 3:1 Methanol:Glacial Acetic Acid.<br>
<b>H&E slide (required) plus paraffin block.</b><br>
<b>Cut Slides:</b> H&E slide (required) plus 3 unstained slides cut at 4-5 microns.<br>
<b>Note for Tech Only clients:</b> Please circle H&E slide.<br>
<b>Note for NYS clients:</b> Cut slides and paraffin block specimens are not accepted.</p>
Flag Active
True
Orderable Test Description
This test uses TCL1 (14q32.1) probes to detect gene abnormalities associated with mature T-cell leukemia, T-cell prolymphocytic leukemia (T-PLL), and adult T-cell leukemia/lymphoma (ATLL).
Orderable Turn Around Time
3-5 Days
Orderable Biomarkers
NY Approved
True
Orderable Biomarkers JSON
{"Fluorescence In Situ Hybridization (FISH)": {"Translocation(s)": ["TCL1"]}}
Keywords string
TCL1, 14q32.1, mature T-cell leukemia, T-cell prolymphocytic leukemia, adult T-cell leukemia TCL1 (14q32.1)
Title URL
tcl1-14q32-1
Clinical Significance
This breakapart probe covers breakpoint regions in the TCL1 (T-cell leukemia) gene cluster on chromosome 14q32 which encompasses TCL1A, TCL1B, and other genes. TCL1 translocations and inversions occur in mature T-cell leukemias including T-cell prolymphocytic leukemia (T-PLL) and adult T-cell leukemia/lymphoma (ATLL). These rearrangements cause gene overexpression due to juxtaposition with TCR-alpha or TCR-beta regulatory elements and contribute to oncogenesis.
Storage and transportation
Use cold pack for transport, making sure cold pack is not in direct contact with specimen. For fresh samples: refrigerate before shipping and ship same day as drawn whenever possible. Do not freeze. NYS clients please provide date and time of collection.