CDKN2A/B (p16) Deletion FISH for ALL | NeoGenomics Laboratories

Code

FSH-PSDA-01BX

Test

Active-Compendium

88377x1 manual or 88374x1 automated

Clinical

Pathology

Bone Marrow Aspirate: 1-2 mL in sodium heparin tube. EDTA tube is acceptable. 
Peripheral Blood: 2-5 mL in sodium heparin tube. EDTA tube is acceptable. 
Fresh, Unfixed Tissue: Tissue in RPMI. 
Fluids: Mix in equal parts with RPMI (specimen volume to RPMI ratio of 1:1). 
Fixed Cell Suspension: A client-prepared fixed cell suspension is acceptable if received in 3:1 Methanol:Glacial Acetic Acid.

Hematologic Diseases

Acute lymphoblastic leukemia (ALL)

Mon, 01/13/2025 - 12:00

Sat, 12/06/2025 - 12:00

FISH

Global

Tech Only

Flag Active

True

Orderable Test Description

This test uses CDKN2A/B (p16) (9p21) and Centromere 9 probes to detect gene abnormalities associated with acute lymphoblastic leukemia (ALL).

Orderable Turn Around Time

3-5 Days

Keywords

Meta Description

The CDKN2A (p16) Deletion for ALL FISH test detects heterozygous and homozygous gene deletions of CDKN2A/B at chromosome 9p21. CDKN2A/B gene deletion is frequently observed in B-cell ALL and is associated with an adverse prognosis in pediatric, adolescent, and adult patients.

Orderable Biomarkers

CDKN2A

NY Approved

True

Orderable Biomarkers JSON

{"Fluorescence In Situ Hybridization (FISH)": {"Deletion(s)": ["CDKN2A"]}}

Keywords string

CDKN2A/B, p15, INK4A, INK4B CDKN2A/B (p16) Deletion FISH for ALL

Title URL

cdkn2a-b-p16-deletion-fish-for-all

Clinical Significance

Loss of the CDKN2A/B gene (also called p16 or pINK4A) at 9p21 is frequently observed in acute lymphocytic leukemia (30-40% of cases) and requires a method more sensitive than cytogenetics (such as FISH) for reliable detection. CDKN2A/B gene deletion is associated with an adverse prognosis in pediatric, adolescent, and adult patients with B-cell ALL (B-cell precursor or BCP-ALL) due to increased risk for relapse, poor response to therapy, lower overall survival, and/or higher incidence of concurrent deletion of other genes. Reports vary whether the impact of heterozygous deletions is as severe as homozygous deletions.

Storage and transportation

Use cold pack for transport, making sure cold pack is not in direct contact with specimen. For fresh samples: refrigerate before shipping and ship same day as drawn whenever possible. Do not freeze. NYS clients please provide date and time of collection.