Code
FSH-PCMF-03BX
Panel
Active-Compendium
88374x5 automated or 88377x5 manual without reflex; with reflex add 88374x4 automated or 88377x4 manual
Clinical
Pathology
<p><b>Bone Marrow Aspirate:</b> 1-2 mL in sodium heparin tube. EDTA tube is acceptable.<br>
<b>Peripheral Blood:</b> 2-5 mL in sodium heparin tube. EDTA tube is acceptable.<br>
<b>Fresh, Unfixed Tissue:</b> Tissue in RPMI.<br>
<b>Bone Marrow/Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides:</b> Minimum 5 slides, labeled with specimen type.<br>
<b>Fluids:</b> Mix in equal parts with RPMI (specimen volume to RPMI ratio of 1:1).<br>
<b>Fixed Cell Suspension:</b> A client-prepared fixed cell suspension is acceptable if received in 3:1 Methanol:Glacial Acetic Acid.<br>
<b>Note for plasma cell enrichment:</b> Will be performed on bone marrow samples unless otherwise directed by the client. Plasma cell enrichment was not validated using peripheral blood; therefore, it is not recommended as a screening specimen unless increased plasma cells are seen on blood smear. Specimens should be received in our laboratory within 72 hours of collection.</p>
<b>Peripheral Blood:</b> 2-5 mL in sodium heparin tube. EDTA tube is acceptable.<br>
<b>Fresh, Unfixed Tissue:</b> Tissue in RPMI.<br>
<b>Bone Marrow/Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides:</b> Minimum 5 slides, labeled with specimen type.<br>
<b>Fluids:</b> Mix in equal parts with RPMI (specimen volume to RPMI ratio of 1:1).<br>
<b>Fixed Cell Suspension:</b> A client-prepared fixed cell suspension is acceptable if received in 3:1 Methanol:Glacial Acetic Acid.<br>
<b>Note for plasma cell enrichment:</b> Will be performed on bone marrow samples unless otherwise directed by the client. Plasma cell enrichment was not validated using peripheral blood; therefore, it is not recommended as a screening specimen unless increased plasma cells are seen on blood smear. Specimens should be received in our laboratory within 72 hours of collection.</p>
Flag Active
True
Orderable Test Description
<p>This FISH Panel is designed to detect key genetic abnormalities associated with plasma cell myeloma (multiple myeloma). It includes the following probes:<br><br>
1p-, 1q+, iso(1q): CDKN2C (1p32), CKS1B (1q21)<br>
+5, hyperdiploidy (5p15)<br>
+9, hyperdiploidy (9q22)<br>
+15, hyperdiploidy (15q22)<br>
13q- (13q14, 13q34)<br>
IgH (14q32)<br>
17p- (TP53 17p13.1, NF1 17q11.2)<br><br>
Reflex Scheme:<br>
Global cases with IgH rearrangement will automatically reflex to the Plasma Cell Myeloma IgH Complex FISH Panel, unless the client has opted out.<br><br>
Test Customization: Probes may be ordered separately, except for +5, +9, and +15, which are combined.</p>
1p-, 1q+, iso(1q): CDKN2C (1p32), CKS1B (1q21)<br>
+5, hyperdiploidy (5p15)<br>
+9, hyperdiploidy (9q22)<br>
+15, hyperdiploidy (15q22)<br>
13q- (13q14, 13q34)<br>
IgH (14q32)<br>
17p- (TP53 17p13.1, NF1 17q11.2)<br><br>
Reflex Scheme:<br>
Global cases with IgH rearrangement will automatically reflex to the Plasma Cell Myeloma IgH Complex FISH Panel, unless the client has opted out.<br><br>
Test Customization: Probes may be ordered separately, except for +5, +9, and +15, which are combined.</p>
Orderable Turn Around Time
3-5 Days
Keywords
Orderable Biomarkers
NY Approved
True
Orderable Biomarkers JSON
{"Fluorescence In Situ Hybridization (FISH)": {"Aneuploidy": ["1p-, 1q+, iso(1q)"], "Hyperdiploidy": ["Chromosome 5", "Chromosome 15", "Chromosome 9"], "Translocation(s)": ["IgH (14q32)"], "Deletion(s)": ["13q", "TP53"]}}
Keywords string
plasmacytoma Plasma Cell Myeloma FISH Panel
Title URL
plasma-cell-myeloma-fish-panel
Clinical Significance
The panel is used for the detection of FISH and chromosome aberrations useful in prognosis in plasma cell myeloma. As malignant plasma cells often have a low proliferation index, conventional cytogenetics frequently yields normal results. When this happens, interphase FISH studies can increase the abnormality detection rate.
TAT Details
Add 3-5 Days if reflexed.
Storage and transportation
Refrigerate specimen. Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. Specimens should be received in our laboratory within 72 hours of collection. NYS clients please provide date and time of collection.