Code
FSH-HGBC-03BX
Panel
Active-Compendium
88374x4 automated. If reflex is added, add 88374x3. Codes may differ if manual analysis is performed.
Clinical
Pathology
<p><b>Bone marrow aspirate:</b> 1-2 mL sodium heparin tube. EDTA tube is acceptable.<br>
<b>Peripheral blood:</b> 2-5 mL sodium heparin tube. EDTA tube is acceptable.<br>
<b>Fresh, unfixed tissue:</b> Tissue in RPMI.<br>
<b>Bone Marrow/ Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides:</b> Minimum 3 slides labeled with specimen type. Three slides are required for the base 3-probe panel, plus 1 slide each if optional 14;18 or 8;14 probes are added.<br>
<b>Fluids:</b>Mix in equal parts with RPMI (specimen volume to RPMI ratio of 1:1).<br>
<b>Fixed Cell Suspension:</b> A client-prepared fixed cell suspension is acceptable if received in 3:1 Methanol:Glacial Acetic Acid.<br>
<b>H&E slide (required) plus paraffin block.</b><br>
<b>Cut Slides:</b> H&E slide (required) plus 8 unstained slides cut at 4-5 microns.
<b>Peripheral blood:</b> 2-5 mL sodium heparin tube. EDTA tube is acceptable.<br>
<b>Fresh, unfixed tissue:</b> Tissue in RPMI.<br>
<b>Bone Marrow/ Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides:</b> Minimum 3 slides labeled with specimen type. Three slides are required for the base 3-probe panel, plus 1 slide each if optional 14;18 or 8;14 probes are added.<br>
<b>Fluids:</b>Mix in equal parts with RPMI (specimen volume to RPMI ratio of 1:1).<br>
<b>Fixed Cell Suspension:</b> A client-prepared fixed cell suspension is acceptable if received in 3:1 Methanol:Glacial Acetic Acid.<br>
<b>H&E slide (required) plus paraffin block.</b><br>
<b>Cut Slides:</b> H&E slide (required) plus 8 unstained slides cut at 4-5 microns.
Flag Active
True
Orderable Test Description
<p>This panel differentiates double-hit or triple-hit lymphomas (which have MYC rearrangements together with BCL2 and/or BCL6 rearrangements) from Burkitt lymphoma or diffuse large B-cell lymphoma. It includes the following probes:<br><br>
MYC (8q24)<br>
MYC/IgH/CEN8 t(8;14)<br>
BCL6 (3q27)<br>
BCL2 (18q21)<br><br>
Optional Reflex:<br>
(1) If MYC (8q24) is positive and MYC/IgH/CEN8 t(8;14) is negative reflex to test for IGK/MYC t(2;8), IGL/MYC t(8;22), and BCL6/MYC t(3;8).<br><br>
Test Customization: Tech Only clients may order probes individually.</p>
MYC (8q24)<br>
MYC/IgH/CEN8 t(8;14)<br>
BCL6 (3q27)<br>
BCL2 (18q21)<br><br>
Optional Reflex:<br>
(1) If MYC (8q24) is positive and MYC/IgH/CEN8 t(8;14) is negative reflex to test for IGK/MYC t(2;8), IGL/MYC t(8;22), and BCL6/MYC t(3;8).<br><br>
Test Customization: Tech Only clients may order probes individually.</p>
Orderable Turn Around Time
3-5 Days
NY Approved
True
Orderable Biomarkers JSON
{"Fluorescence In Situ Hybridization (FISH)": {"Amplification(s)": ["MYC"], "Rearrangement(s)": ["BCL2", "BCL6", "MYC-IgH"]}}
Keywords string
Large B-cell Lymphoma, BCL2, BCL6, Burkitt lymphoma, diffuse large B-cell lymphoma. High-Grade/Large B-Cell Lymphoma Panel
Title URL
high-grade-large-b-cell-lymphoma-panel
Storage and transportation
Use cold pack for transport, making sure cold pack is not in direct contact with specimen. NYS clients please provide date and time of Collection.