Code
FSH-BCLT-01BX
Test
Active-Compendium
88377x1 manual or 88374x1 automated
Clinical
Pathology
<p><b>Bone Marrow Aspirate:</b> 1-2 mL in sodium heparin tube. EDTA tube is acceptable.<br>
<b>Peripheral Blood:</b> 2-5 mL in sodium heparin tube. EDTA tube is acceptable.<br>
<b>Fresh, Unfixed Tissue:</b> Tissue in RPMI.<br>
<b>Bone Marrow/Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides:</b> Minimum 1 slide, labeled with specimen type.<br>
<b>Fluids:</b> Mix in equal parts with RPMI (specimen volume to RPMI ratio of 1:1).<br>
<b>Fixed Cell Suspension:</b> A client-prepared fixed cell suspension is acceptable if received in 3:1 Methanol:Glacial Acetic Acid.<br>
<b>H&E slide (required) plus paraffin block.</b><br>
<b>Cut Slides:</b> H&E slide (required) plus 3 unstained slides cut at 4-5 microns.<br>
<b>Note for Tech Only clients:</b> Please circle H&E slide.</p>
<b>Peripheral Blood:</b> 2-5 mL in sodium heparin tube. EDTA tube is acceptable.<br>
<b>Fresh, Unfixed Tissue:</b> Tissue in RPMI.<br>
<b>Bone Marrow/Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides:</b> Minimum 1 slide, labeled with specimen type.<br>
<b>Fluids:</b> Mix in equal parts with RPMI (specimen volume to RPMI ratio of 1:1).<br>
<b>Fixed Cell Suspension:</b> A client-prepared fixed cell suspension is acceptable if received in 3:1 Methanol:Glacial Acetic Acid.<br>
<b>H&E slide (required) plus paraffin block.</b><br>
<b>Cut Slides:</b> H&E slide (required) plus 3 unstained slides cut at 4-5 microns.<br>
<b>Note for Tech Only clients:</b> Please circle H&E slide.</p>
Flag Active
True
Orderable Test Description
This test uses BCL2 (18q21) probes to detect gene rearrangements associated with follicular lymphoma and diffuse large B-cell lymphoma (DLBCL).
Orderable Turn Around Time
3-5 Days
Keywords
Orderable Biomarkers
NY Approved
True
Orderable Biomarkers JSON
{"Fluorescence In Situ Hybridization (FISH)": {"Structural Rearrangement(s)": ["BCL2"]}}
Keywords string
BCL2 FISH, B-cell lymphoma BCL2 (18q21)
Title URL
bcl2-18q21
Clinical Significance
This BCL2 break-apart probe enables detection of BCL2 rearrangements at 18q21 irrespective of the partner gene. Detection of BCL2 rearrangement aids in diagnosis and classification of follicular lymphoma (FL), diffuse large B-cell lymphoma (DLBCL), and other aggressive B-cell lymphomas in conjunction with clinical, morphologic, and flow cytometric data.
The most frequent rearrangement partner is IgH (14q32). Rarely, variant translocations involve the light chain genes Ig lambda (22q11) or Ig kappa (2p12), AFF3 (2q11), or the mu switch region. BCL2 functions in anti-apoptosis and its overexpression is implicated in oncogenesis.
Storage and transportation
Use cold pack for transport, making sure cold pack is not in direct contact with specimen. For fresh samples: refrigerate before shipping and ship same day as drawn whenever possible. Do not freeze. NYS clients please provide date and time of collection.