Code
APT-NUTX-01AX
Test
Active-Compendium
88342x1 or 88341x1
Clinical
Pathology
A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type or one (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody ordered.
Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Flag Active
True
Orderable Test Description
Expression of nuclear protein in testis (NUT) is generally confined to the germ cells of the testis and ovary. NUT midline carcinomas are aggressive tumors with non-diagnostic morphology that overlaps with many other poorly differentiated tumors, but are characterized by rearrangement of the NUTM1 (NUT) gene at chromosome 15q14 with BRD4/3 (and rarely NSD3, ZNF532 or ZNF592), which causes NUT protein overexpression. IHC staining with NUT antibody may serve as a diagnostic alternative to FISH or molecular confirmation of 15q14 rearrangement. However, NUT overexpression by IHC may also be seen in other diseases NUTM1 rearrangement, such as porocarcinoma with YAP1-NUTM1 gene fusion (PMID: 32436169), so a diagnosis of NUT midline carcinoma should not be based on NUT IHC positivity alone.
Orderable Turn Around Time
1 Day
Extra Note
Use cold pack for transport, making sure cold pack is not in direct contact with specimen. NYS clients please provide date and time of Collection.
Orderable Biomarkers
NY Approved
True
Orderable Biomarkers JSON
{"Immunohistochemistry (IHC)": {"Expression": ["NUT"]}}
Keywords string
NUT, nutm1 NUT
Title URL
nut