Code
NTG-DRBX-02CX
Profile
Active-Compendium
81457x1, 88377x8, 88360x1. Add 81287x1 if ordering MGMT Promoter Methylation Analysis.
81479x1, 88377x8, 88360x1. If sample is insufficient to produce RNA fusion results but DNA SNV/indel and/or CNV results are reported, 81479x1 still applies. If only RNA fusion results are reported, use 81445x1 instead of 81479x1. (SEE NOTES)
Clinical
Oncology,Pathology
FFPE solid tumor tissue: Minimum surface area 10mm2 with 20% tumor content. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.
Paraffin block: Preferred.
Cut slides: Send 25 unstained sections cut at 5 microns plus one H&E slide (which NeoGenomics will keep). No additional slides are needed if ordering MGMT Promoter Methylation Analysis.
Paraffin block: Preferred.
Cut slides: Send 25 unstained sections cut at 5 microns plus one H&E slide (which NeoGenomics will keep). No additional slides are needed if ordering MGMT Promoter Methylation Analysis.
Flag Active
True
Orderable Test Description
The NeoTYPE® DNA & RNA – Brain Profile uses targeted next‑generation sequencing (NGS) to detect single nucleotide variants, insertions/deletions, and gene fusions across <b>83 unique genes (62 DNA and 28 RNA</b>; overlapping targets included). The profile also assesses microsatellite instability <b>(MSI)</b> and tumor mutational burden <b>(TMB)</b>. <br><br>
<b>NGS – SNVs / Indels (62 genes + MSI and TMB)</b><br>
Reflex rule: A microsatellite instability (MSI) NGS result of “indeterminate” will create a reflex to MSI by PCR as long as the tumor percentage is ≥40% and paired normal tissue is available.<br><br>
<b>RNA Fusions (28 genes)</b><br><br>
<b>FISH – CNVs (9)</b><br>
1p/19q co‑deletion, +7/‑10 (trisomy 7, monosomy 10), CDKN2A (p16) deletion, EGFR amplification, MET amplification, MYCN amplification, PDGFRA amplification, PTEN deletion<br>
(FISH is global only)<br><br>
<b>IHC (1 biomarker)</b><br>
PD‑L1 LDT (tech‑only available)<br><br>
<b>Test customization:</b><br>
MGMT Promoter Methylation Analysis by PCR<br><br>
<b>Legacy Test information: </b><br>
As of May 12, 2025, this test serves as replacement for the following test(s) that are no longer offered:<br>
* Brain NGS Fusion Panel</p>
<b>NGS – SNVs / Indels (62 genes + MSI and TMB)</b><br>
Reflex rule: A microsatellite instability (MSI) NGS result of “indeterminate” will create a reflex to MSI by PCR as long as the tumor percentage is ≥40% and paired normal tissue is available.<br><br>
<b>RNA Fusions (28 genes)</b><br><br>
<b>FISH – CNVs (9)</b><br>
1p/19q co‑deletion, +7/‑10 (trisomy 7, monosomy 10), CDKN2A (p16) deletion, EGFR amplification, MET amplification, MYCN amplification, PDGFRA amplification, PTEN deletion<br>
(FISH is global only)<br><br>
<b>IHC (1 biomarker)</b><br>
PD‑L1 LDT (tech‑only available)<br><br>
<b>Test customization:</b><br>
MGMT Promoter Methylation Analysis by PCR<br><br>
<b>Legacy Test information: </b><br>
As of May 12, 2025, this test serves as replacement for the following test(s) that are no longer offered:<br>
* Brain NGS Fusion Panel</p>
Orderable Turn Around Time
14 Days
Keywords
Orderable Biomarkers
NY Approved
True
Orderable Biomarkers JSON
{"DNA Sequencing": {"SNVs + Indels": ["AKT1", "APC", "ATRX", "BAP1", "BCOR", "BCORL1", "BRAF", "CDK6", "CDKN2A", "CDKN2B", "CIC", "CTNNB1", "DICER1", "EED", "EGFR", "EPCAM", "ERBB2", "ERBB4", "FGFR1", "FGFR2", "FGFR3", "FUBP1", "GNA11", "GNAQ", "H3-3A", "H3C3", "HRAS", "IDH1", "IDH2", "KDM6A", "KRAS", "MAP2K1", "MET", "MLH1", "MSH2", "MSH6", "MYC", "MYCN", "NF1", "NF2", "NOTCH1", "NRAS", "PDGFRA", "PIK3CA", "PMS2", "PTCH1", "PTEN", "RB1", "SETD2", "SF3B1", "SMAD4", "SMARCA4", "SMARCB1", "SMO", "SRC", "SUFU", "SUZ12", "TERT", "TP53", "TSC1", "TSC2", "VHL"], "Genomic Signatures": ["MSI", "TMB"], "Promoter Methylation": ["MGMT"]}, "RNA Sequencing": {"Fusions": ["ALK", "BRAF", "CIC", "EGFR", "EML4", "ETV6", "EWSR1", "FGFR1", "FGFR2", "FGFR3", "FUS", "KIAA1549", "MAML2", "MET", "MN1", "MYB", "MYBL1", "NTRK1", "NTRK2", "NTRK3", "PRKCA", "RAF1", "ROS1", "STAT6", "TACC3", "TFG", "YAP1", "ZFTA"], "Splice Variant": ["EGFR"]}, "Immunohistochemistry (IHC)": {"Expression": ["PD-L1"]}, "Fluorescence In Situ Hybridization (FISH)": {"Amplification(s)": ["EGFR", "MET", "MYCN", "PDGFRA"], "Deletion(s)": ["1p19q", "p16", "PTEN"], "Trisomy": ["Chromosome 7"], "Monosomy": ["Chromosome 10"]}}
Keywords string
CNS, WHO, medulloblastoma, embryonal, cytoma, astrocytoma, glioma, glioneuronal, ependymoma, meningioma, pharyngioma, Brain NGS Fusion Panel NeoTYPE® DNA & RNA - Brain
Title URL
neotype-dna-rna-brain
TAT Details
NGS: 14 Days
FISH: 7 Days
IHC: Global: 2 Days
Tech-Only (stain
only): 24 Hours
Potential Reflex Testing (Additional Time):
MSI by PCR: 5 Days
Storage and transportation
Use cold pack for transport, making sure cold pack is not in direct contact with specimen. NYS clients please provide date and time of Collection.